Ovalbumin was first isolated through successive salt precipitations in 1889, and the procedure was later improved in the first part of the 20th century. The standard purification procedures were labor intensive and difficult to mechanize, which means that large-scale production of pure Ovalbumin was not feasible. With the development of a purification procedure using chromatography techniques, we are able to produce extremely pure Ovalbumin in commercial volumes. Ovalbumin is very similar in amino acid content to bovine serum albumin (BSA) and can be an excellent substitute for BSA.
Ovalbumin is a key reference protein for immunization and biochemical studies and can be used as follows:
- As a standard in the investigation of composition, physical properties, and structure of proteins – in high purity crystalline form.
- As a blocking agent in immunohistochemistry and in western blots.
- For the detection of anti-hemoglobin monoclonal antibodies in enzyme-linked immunosorbent assays (ELISA).
- As a protein standard in molecular weight determination by SDS-PAGE and size exclusion chromatography.
In addition, Ovalbumin may be used in cell culture systems and in diagnostics to stabilize enzymes and hormones that would otherwise lose their functional integrity. It is also an ideal reference protein for immunological and biochemical studies, where it serves as an effective carrier and stabilizer. An important advantage of Ovalbumin is that it’s free from the risk of diseases associated with bovine and human-derived albumins. Ovalbumin also originates from a safer starting material than the other albumins and is processed to a pure form under more sanitary conditions.
- Selling Unit: Kilograms(Kg)